Publications

TB pathogenesis, persistence, and animal models:

Karakousis PC, Yoshimatsu T, Lamichhane L, Woolwine SC, Nuermberger EL, Grosset J, Bishai WR. Dormancy phenotype displayed by extracellular Mycobacterium tuberculosis within artificial granulomas in mice. J Exp Med. 2004; 200:647-57. PMCID: PMC2212740

Jain SK, Hernandez-Abanto, Cheng Q-J, Singh P, Ly LH, Klinkenberg LG, Morrison NE, Converse PJ, Nuermberger EL, Grosset J, McMurray DN, Karakousis PC, Lamichhane G, Bishai WR. Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs compared with mice. J Infect Dis. 2007;195:1634-42. PMID: 17471433

Williams EP, Lee JH, Bishai WR, Colantuoni C, Karakousis PC. Mycobacterium tuberculosis SigF regulates genes encoding cell wall-associated proteins and directly regulates the transcriptional regulatory gene phoY1. J Bacteriol. 2007;189:4234-42. PMCID: PMC1913405

Lee, J-H, Karakousis PC, Bishai WR. Characterization of sigma factor regulation in Mycobacterium tuberculosis by SigB and SigF. J Bacteriol. 2008;190:699-707. PMCID: PMC2223694

Karakousis PC, Williams EP, Bishai WR. Altered expression of isoniazid-regulated genes in drug-treated dormant Mycobacterium tuberculosis. J Antimicrob Chemother. 2008;61:323-31. PMID: 18156607

Klinkenberg L, Sutherland L, Bishai WR, Karakousis PC. Metronidazole lacks activity against Mycobacterium tuberculosis in an in vivo hypoxic granuloma model of latency. J Infect Dis. 2008;198:275-83. PMCID: PMC2647800

Converse PJ, Karakousis PC, Klinkenberg LG, Kesavan AK, Ly LH, Allen SS, Grosset JH, Jain SK, Lamichhane G, Manabe YC, McMurray DN, Nuermberger EL, Bishai WR. The role of the DosR/DosS two-component regulatory system in Mycobacterium tuberculosis virulence in three animal models. Infect. Immun. 2009;77:1230-37. PMCID: PMC2643651

Rifat D, Bishai WR, Karakousis PC. Phosphate depletion: A novel trigger for Mycobacterium tuberculosis persistence. J Infect Dis. 2009;200:1126-35. PMID: 19686042

Rao NA, Albini TA, Kumaradas M, Pinn ML, Fraig MM, Karakousis PC. Experimental ocular tuberculosis in guinea pigs. Arch. Ophthalmol. 2009;127:1162-6. PMCID: PMC3062475

Converse PJ, Eisenach KD, Theus SA, Nuermberger EL, Tyagi S, Ly LH, Geiman DE, Guo H, Nolan ST, Akar NC, Klinkenberg LG, Gupta R, Lun S, Karakousis PC, Lamichhane G, McMurray DN, Grosset JH, Bishai WR. The impact of mouse passaging of Mycobacterium tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models. PLoS One. 2010;5:e10289. PMCID: PMC2858211

Klinkenberg LG, Lee J-H, Bishai WR, Karakousis PC. The stringent response is required for full virulence of Mycobacterium tuberculosis in guinea pigs. J Infect Dis. 2010;202:1397-1404. PMCID: PMC2949470

Abomoelak B, Ward SK, Marcus S, Karakousis PC, Steinberg H, Talaat AM. Characterization of a novel heat shock protein (Hsp22.5) involved in the pathogenesis of Mycobacterium tuberculosis. J Bacteriol. 2011;193:3497-505. PMCID: PMC3133320

Be NA, Klinkenberg LG, Bishai WR, Karakousis PC, Jain SK. Strain-dependent CNS dissemination in guinea pigs after Mycobacterium tuberculosis aerosol challenge. Tuberculosis (Edinb). 2011;91:386-9. PMCID: PMC3172348

Thayil SM, Morrison N, Schechter N, Rubin H, Karakousis PC. The role of the novel exopolyphosphatase MT0516 in Mycobacterium tuberculosis drug tolerance and persistence. PLoS One. 2011;6:e28076. PMCID: PMC3221697

Thayil SM, Albini TA, Nazari H, Moshfeghi AA, Parel J-MA, Rao NA, Karakousis PC. Local Ischemia and Increased Expression of Vascular Endothelial Growth Factor Following Ocular Dissemination of Mycobacterium tuberculosis. PLoS One. 2011;6:e28383. PMCID: PMC3230586

Singh PP, Smith VL, Karakousis PC, Schorey JS. Exosomes isolated from M. tuberculosis infected cells can induce migration and recruitment of host immune cells in vivo. J Immunol. 2012;189:777-85. PMCID: PMC3685416

Thayil SM, Ho Y-C, Bollinger RC, Blankson JN, Siliciano RF, Karakousis PC*, Page KR. Mycobacterium tuberculosis complex enhances HIV infection susceptibility of CD4 T cells to HIV through a TLR2-mediated pathway. PLoS One. 2012;7:e41093. *Corresponding author. PMCID: PMC3402510

Bisson GP, Mehaffy C, Broeckling C, Prenni J, Rifat D, Lun D, Burgos M, Weissman D, Karakousis PC, Dobos KM. Upregulation of the phthiocerol dimycocerosate biosynthetic pathway by rifampicin-resistant, rpoB-mutant Mycobacterium tuberculosis. J Bacteriol. 2012;194:6441-52. PMCID: PMC3497527

Klinkenberg LG, Karakousis PC. Rv1894c is a novel hypoxia-induced nitronate monooxygenase required for Mycobacterium tuberculosis virulence. J Infect Dis. 2013;207:1525-34. PMID: 23408846

Subbian S, O'Brien P, Kushner NL, Yang G, Tsenova L, Peixoto B, Bandyopadhyay N, Bader JS, Karakousis PC, Fallows D, Kaplan G. Molecular immunologic correlates of spontaneous latency in a rabbit model of pulmonary tuberculosis. Cell Commun Signal . 2013;11:16. PMCID: PMC3598925

Chuang, Y-M, Belchis DA, Karakousis PC. The polyphosphate kinase gene ppk2 is required for Mycobacterium tuberculosis inorganic polyphosphate regulation and virulence. MBio. 2013;4:e00039-13. PMCID: PMC3663568

SkerryC, Pokkali S, PinnML, Be NA, Harper J, Karakousis PC, Jain SK. Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in guinea pigs. PLoS One. 2013; 8:e66310. PMCID: PMC3679071. PMID: 23776655

Lanzas F, Karakousis PC, Sacchettini JC, Ioerger TR. Multidrug-resistant tuberculosis in Panama is driven by clonal expansion of an MDR-TB strain related to the KZN XDR-TB strain from South Africa. J Clin Microbiol. 2013;51:3277-85. PMID: 23884993

Subbian S, Bandyopadhyay N, Tsenova L, O Brien P, Khetani V, Kushner NL, Peixoto B, Soteropoulos P, Bader JS, Karakousis PC, Fallows D, Kaplan G. Early innate immunity determines outcome of Mycobacterium tuberculosis pulmonary infection in rabbits. Cell Commun Signal. 2013;11:60. PMID: 23958185

Nazari H, Karakousis PC, Rao NA. Replication of Mycobacterium tuberculosis in Retinal Pigment Epithelium (In press, JAMA Ophthalmol).2014;132:724-9. PMID: 24723139

Dutta N, Bandyopadhyay N, Veeramani B, Lamichhane G, Karakousis PC*, Bader J. Systems biology-based identification of Mycobacterium tuberculosis persistence genes in mouse lungs. MBio. 2014;5: *Co-corresponding author. PMID: 24549847

Dutta NK, Illei P, Jain S, Karakousis PC. Characterization of a novel necrotic granuloma model of latent tuberculosis infection and reactivation in mice. Am J Pathol. 2014;184:2045-55. PMID: 24815353

Rifat D, Karakousis PC. Differential regulation of the two-component regulatory system senX3-regX3 in Mycobacterium tuberculosis. Microbiology. 2014;160:1125-33. PMID: 24722908

Heaton B, Barkan D, Bongiorno P, Karakousis PC, Glickman MS. Deficiency of double strand DNA break repair does not impair Mycobacterium tuberculosis virulence in multiple animal models of infection. Infect Immun. 2014;82:3177-85. PMID: 24842925

Rifat D, Belchis DA, Karakousis PC. senX3-independent contribution of regX3 to Mycobacterium tuberculosis virulence. BMC Microbiol. 2014;14:265. PMID: 25344463

TB Chemotherapy in Animal Models:

Ahmad Z, Klinkenberg LG, Pinn ML , Fraig MM, Peloquin CA, Bishai WR, Nuermberger EL, Grosset J, Karakousis PC. Biphasic kill curve of isoniazid reveals the presence of drug-tolerant, not drug-resistant, Mycobacterium tuberculosis in the guinea pig. J Infect Dis. 2009;200:1136-43. PMID: 19686043

Ahmad Z, Nuermberger EL, Tasneen R, Pinn ML, Williams KN, Peloquin CA, Grosset J, Karakousis PC. Comparison of the ‘Denver regimen’ against acute tuberculosis in the mouse and guinea pig. J Antimicrob Chemother. 2010;65:729-34. PMCID: PMC2837551

Ahmad Z, Pinn ML, Nuermberger EL, Peloquin CA, Grosset J, Karakousis PC. The potent bactericidal activity of streptomycin in the guinea pig model of tuberculosis ceases due to the presence of persisters. J Antimicrob Chemother.2010;65:2172-5. PMCID: PMC2941674

Ahmad Z, Fraig MM, Bisson GP, Nuermberger EL, Grosset JH, Karakousis PC. Dose-dependent activity of pyrazinamide in animal models of intracellular and extracellular tuberculosis. Antimicrob Agents Chemother. 2011;55:1527-32. PMCID: PMC3067197

Ahmad Z, Fraig MM, Pinn ML, Tyagi S, Nuermberger EL, Grosset JH, Karakousis PC. Effectiveness of tuberculosis chemotherapy correlates with resistance to Mycobacterium tuberculosis infection in animal models. J Antimicrob Chemother. 2011;66:1560-6. PMCID: PMC3112031

Dutta NK, Illei PB, Peloquin CA, Pinn ML, Mdluli KE, Nuermberger EL, Grosset JH, Karakousis PC. Rifapentine is not more active than rifampin against chronic tuberculosis in guinea pigs. Antimicrob Agents Chemother. 2012;56:3726-31. PMCID: PMC3393407

Rosenthal IM, Tasneen R, Peloquin CA, Zhang M, Almeida D, Mdluli KE, Karakousis PC, Grosset JH, Nuermberger EL. Dose-ranging comparison of rifampin and rifapentine in two pathologically distinct murine models of tuberculosis. Antimicrob Agents Chemother. 2012;56:4331-40. PMCID: PMC3421552

Dutta NK, Sultan A, Peloquin CA, Karakousis PC. Preliminary Pharmacokinetic Study of Repeated Doses of Rifampin and Rifapentine in Guinea pigs. Antimicrob Agents Chemother. 2013;57:1535-7. PMCID: PMC3591898

Dutta NK, Pinn ML, Zhao M, Rudek MA, Karakousis PC. Thioridazine lacks bactericidal activity in an animal model of extracellular tuberculosis. J Antimicrob Chemother. 2013;68:1327-30. PMCID: PMC3654222

Dutta NK, Alsultan A, Gniadek TJ, Belchis DA, Pinn ML, Mdluli KE, Nuermberger EL, Peloquin CA, Karakousis PC. Potent rifamycin-sparing regimen cures guinea pig tuberculosis as rapidly as the standard regimen. Antimicrob Agents Chemother. 2013; 57:3910-6. PMCID: PMC3719725

Dutta NK, Pinn ML, Karakousis PC. Reduced emergence of isoniazid resistance with concurrent use of thioridazine against acute murine tuberculosis. Antimicrob Agents Chemother. 2014;58:4048-53. PMCID: PMC4068531

Skerry C, Pinn ML, Bruiners N, Pine R, Gennaro ML, Karakousis PC. Simvastatin increases the in vivo activity of the first-line TB regimen. J Antimicrob Chemother. 2014;69:2453-7. PMCID: PMC4184365

Dutta NK, Pinn ML, Karakousis PC. Sterilizing activity of thioridazine in combination with the first-line regimen against acute marine TB. Antimicrob Agents Chemother. 2014;58:5567-9. PMCID: PMC4135856

Dutta NK, Karakousis PC. PA-824 is as effective as isoniazid against latent tuberculosis infection in C3HeB/FeJ mice. (In Press, Int J Antimicrob Agents) PMID: 25270632

Diagnostics for TB and Drug Resistance:

Zhou A, Nawaz M, Xue X, Karakousis PC, Yao Y, Xu J. Molecular genotyping of Mycobacterium tuberculosis in Xi'an, China using MIRU-VNTR typing system. Int J Tuberc Lung Dis. 2011;15:517-22. PMID: 21396212

Chia B-S, Lanzas F, Rifat D, Herrera A, Kim EY, Sailer C, Torres-Chavolla E, Narayanaswamy P, Einarsson V, Bravo J, Pascale JM, Ioerger TR, Sacchettini JC, Karakousis PC. Use of Multiplex Allele-Specific Polymerase Chain Reaction (MAS-PCR) to Detect Multidrug-Resistant Tuberculosis in Panama. PLoS One . 2012;7:e40456. PMCID: PMC3391257

Zhou A, Ni J, Xu Z, Wang Y, Lu S, Sha W, Karakousis PC, Yao Y-F. Application of 1H-NMR spectroscopy-based metabolomics to sera of tuberculosis. J Proteome Res. 2013;12:4642-9. PMID: 23980697

Reviews/Book Chapters:

Karakousis PC, Bishai WR, Dorman SE. Mycobacterium tuberculosis cell envelope lipids and the host immune response. Cell Microbiol. 2004;6:105-116. PMID: 14706097

Karakousis PC, Chaisson RE. Mycobacterial infections and HIV infection. In: Fishman's Pulmonary Diseases and Disorders, 4th edition, Fishman AP, Elias JA, Fishman JA, Grippi MA, Senior RM, Pack AI, ed. New York: McGraw Hill, 2008:2487-2497.

Karakousis PC. Mechanisms of Action and Resistance of the Antimycobacterial Agents. In: Antimicrobial Drug Resistance, Mayers D, ed. New York: Humana Press, 2009:271-291.

Kolyva A, Karakousis PC. Old and New TB Drugs: Mechanisms of Action and Resistance. In: Mycobacterium tuberculosis/Book 2, Cardona P-J, ed. InTech, 2011.

Dutta NK, Mazumdar K, Dastidar SG, Karakousis PC, Amaral L. New Patentable Use of an Old Neuroleptic Compound Thioridazine to Combat Tuberculosis: A Gene Regulation Perspective. Recent Pat Antiinfect Drug Discov . 2011;6:128-38. PMID: 21517741

Dutta NK, Karakousis PC. Tuberculosis (TB) Chemotherapy: Present Situation, Possible Solutions, and Progress towards a TB-free world. Indian J Med Microbiol. 2012;30:261-3. PMID: 22885189

Dutta NK, Karakousis PC. Latent tuberculosis infection: Myths, models, and molecular mechanisms. Microbiol Mol Biol Rev. 2014;78:343-371. PMCID: PMC4187682

Dutta NK, Karakousis PC. Thioridazin for treatment of tuberculosis: Promises and putfalls. (In press, Tuberculosis) PMID: 25293998

Kana BD, Karakousis PC, Parish T, Dick T. Future target-based drug discovery for tuberculosis? (In press, Tuberculosis)